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February 9, 2010
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Shark Test


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Scientists have developed a new weapon in the war against poaching great white sharks. As this ScienCentral News video reports, this new test will make it easier to spot sales of the endangered species.

Great White Myth

Thanks to movies like Jaws, most people think of great white sharks as vicious hunters. But the truth is, you're more likely to get killed by a falling coconut than a shark, and humans are hunting the great whites right out of existence, selling everything from their teeth (for jewelry) to their fins (for soup).

"Just in the Northwest Atlantic alone, the population of the great white has plummeted by 79 percent just since 1986," says Ellen Pikitch, director of ocean strategy for the Wildlife Conservation Society (WCS). "It's ironic that great white sharks have been thought of as being man-eaters. They've been maligned in the press, and what is really happening is that humans are hunting the great white out of existence, and not the other way around."





Great whites are among the world's most legally protected shark species, but the laws are hard to enforce, partially because of the lack of means to identify the sharks from their marketed parts. "Oftentimes great white sharks appear just as either fins alone, cartilage pills or in ground up flesh, possibly even mixed in with other species," explains Pikitch. "So, it's really hard to tell from those shark products whether or not you've got a great white shark."

great white shark
image: Wildlife Conservation Society
So Pikitch and her WCS team, along with researchers from Nova Southeastern University in Florida, came up with a way to better protect these toothy beasts by developing a faster way to find out whether great whites are being hunted illegally for their meat—a DNA test.

DNA Taste Test





Most people are familiar with DNA tests from criminal investigations, and this test is similar. But in this case, scientists must be able to rely on DNA to distinguish one shark species from another.




Writing in the August issue of the journal Conservation Genetics, Pikitch reported that the new test not only cut test time from three days to 24 hours, but also is very accurate. "There are other genetic tests that have been used to detect other species of wildlife, but what’s different about this one is that it’s very sensitive, it is very inexpensive to use, and it’s very rapid," says Pikitch. "This is the first genetic test that has ever come out that enables the person to tell great white sharks definitively, even from the tiniest bit of tissue."

The researchers used a technique called polymerase chain reaction, or PCR. The new test uses DNA from shark cell nuclei and mitochondria, the "powerhouses" of the cells. The team tested 53 samples of great white and 68 samples of other shark species from all over the world. None of the tests wrongly confused great whites with other sharks.

sand tiger shark
Sand tiger shark.
image: Wildlife Conservation Society
"In order to know that this test is really valid, really accurate, you need to be able to test it looking for false positives and false negatives against other closely-related shark species," says Pikitch. "We are able to distinguish between a false negative and a test that wasn’t done properly. And that’s one of the beauties of this test that makes it really unique—that we know for sure whether we have a failure of the test itself, or there just aren’t any great white sharks in the sample.”

The test can detect the presence of great white DNA in a mixture of DNA from up to ten different commercially fished shark species. Pikitch points out that although her test is just for great white sharks, the method could be streamlined and used to develop similar tests for other species.

This research is funded by the Wildlife Conservation Society, the Roe Foundation, the David and Lucile Packard Foundation and the Curtis and Edith Munson Foundation.


 
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